Coding
Part:BBa_K2933122:Design
Designed by: Xueqing Fu Group: iGEM19_TJUSLS_China (2019-09-14)
GST+Linker+Fla.103
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 774
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 774
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1256
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 774
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 774
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 85
Design Notes
Fla.103 is codon optimized for BL21(DE3) E. coli.
Source
NCBI.